• HELBEN ISMAT MOHAMMAED Dept. of Plant Protection,Collage of Agriculture Engineering Sciences, University of Duhok
  • MOHAMMED SAEED MIRZA Dept. of Plant Protection,Collage of Agriculture Engineering Sciences, University of Duhok
  • FEYROZ RAMADAN HASSAN Dept. of Plant Protection,Collage of Agriculture Engineering Sciences, University of Duhok
Keywords: B. bassiana, gelatin, preservation method, microbial baits, ant.


Beauveria bassiana is an important entomopathogenic fungus that used as a biocontrol agent of insect
pests. Maintaining and preserving B. bassiana cultures is essential for the effective evaluation of its
potential as microbial agent against insect pest, for biodiversity studies and also for exchange of fungal
material between laboratories. In the present work we evaluated the suitability of four preservation
materials based on gelatin and rice to maintain the viability of B. bassiana to be used as baits for insect’s
control. The gelatin amended with sugar recorded the maximum viability after 70 days of storage as
98.3% compared to 63.3% on rice media. Effective control of 80% of the ants was observed after 10 days
of feeding on gelatin amended with sugar and rice bait. The results demonstrated that treatment with
formulations containing conidia of B. bassiana presents insecticidal activity against ant in addition of
acting as preservation materials.


Download data is not yet available.


Abd-Elsalam KA, Yassin MA, Moslem M, Bahkali
AH, De Wit PJGM, Mckenzie EHC,
Stephenson SL, Cai L and Hyde KD (2010).
Culture collections, the new herbaria for
fungal pathogens. Fungal diversity 45(1):21-
Akello J, Dubois T, Coyne D & Kyamanywa S
(2009). The effects of Beauveria bassiana
dose and exposure duration on colonization
and growth of tissue cultured banana (Musa
sp.) plants. Biol. Control 49: 6-10.
Aparecido CC and Camilo CM (2013). Comparação
de métodos para a preservação de fungos do
gênero Colletotrichum em laboratório.
Biológico, 75, pp17-22.
Ayala-Zermeño MA, Gallou A, Berlanga-Padilla
AM, Andrade-Michel GY, Rodríguez-
Rodríguez JC, Arredondo-Bernal HC and
Montesinos-Matías R (2017). Viability, purity,
and genetic stability of entomopathogenic
fungi species using different preservat ion
Beloti IF, Juliatti BCM and Juliatti FC (2017).
Evaluation of the Gelatin Technique for the
Preservation of Phytopathogenic Fungi.
Biosci. J., V. 33, N. 4, p. 923-932.
Bextine, B. R., and H. G.Thorvilson. 2002. Field
applications of bait-for-mulated Beauveria
bassiana alginate pellets for biological control
of the red imported fire ant (Hymenoptera:
Formicidae). Environ. Entomol. 31: 746–752.
Daza FFF, Roman GR, Rodriguez MV, Vargas IAG,
Heano HC, Cereda MP and Mulet RAC
(2019). Spores of Beauveria bassiana and
Trichoderma lignorum as a bioinsecticide for
the control of Atta cephalotes. Biol Res 52:51. Fuxa, J. R., and A. R. Richter. 2004. Effects of soil
moisture and composition and fungal isolate
on prevalence of Beauveria bassiana in
laboratory col-onies of the red imported fire
ant (Hymenoptera: Formicidae). Environ.
Entomol. 33: 975–981.
Hassan FR (2019). Selective Isolation and Biomass
Production of Beauveria bassiana and its
Virulence to Squash Beetle Epilachna
chrysomelina F. Ph.D dissertation,College of
Agricultural Engineering Sciences / University
of Duhok 165pp.
Immediato D, Iatta R, Camarda A, Giangaspero A,
Capelli G, Figueredo LA, Otranto D and
Cafarchia C. (2017). Storage of Beauveria
bassiana Conidia Suspension: A Study
Exploring the Potential Effects on Conidial
Viability and Virulence against Dermanyssus
gallinae De Geer, 1778 Acari: Dermanyssidae.
Annals of Biological Sciences, 5 (2): 69-76.
Li J, Guo Q, Lin M, Jiang L, Ye J, Chen D, Li Z, Dai
J and Han S (2016). Evaluation of a New
Entomopathogenic Strain of Beauveria
bassiana and a New Field Delivery Method
against Solenopsis invicta. PLoS ONE 11(6).
Meyling NV & Eilenberg J (2007). Ecology of the
entomopathogenic fungi Beauveria bassiana
and Metarhizium anisopliae in temperate
agroecosystems: potential for conservation
biological control. Biological control 43: 145–
Padmini PCP and Padmaja V (2010). Impact of
different relative humidities on in vitro growth
and sporulation of entomopathogenic fungal
isolates of Beauveria species. Int J Pharm Biol
Sci Arch.1:355–359.
Rojas MG, Elliott RB and Morales-Ramos JA (2018).
Mortality of Solenopsis invicta Workers
(Hymenoptera: Formicidae) After Indirect
Exposure to Spores of Three
Entomopathogenic Fungi. Journal of Insect
Science, 18(3): 20; 1–8.
Roy H. E., Brodie E. L., Chandler D., Goettel S. M.,
Pell K. J., Wajnberg E., et al. (2010). Deep
space and hidden depths: understanding the
evolution and ecology of fungal
entomopathogens. Biocontrol 55 1–6.
Siebeneicher SR, Vinson DB and Kenerley CM
(1992). Infection of the red imported fire ant
by Beauveria bassiana through various routes
of exposure. Journal of Invertebrate
Pathology, 59: 280-285.
Šimková J (2009). Influence of different storage
conditions on vitality and virulence of
Beauveria bassiana spores. Journal of
Agrobiology, 26 (2): 75-81.
How to Cite
MOHAMMAED, H. I., MIRZA, M. S., & HASSAN, F. R. (2020). PRESERVATIVE MEDIA FOR Beauveria bassiana (BALS.) VUILL STORAGE. Journal of Duhok University, 23(2), 1-6.
Agriculture and Veterinary Science