• RAWA NOORI AWAD Dept. of Microbiology and Parasitology, College of veterinary medicine, University of Duhok
  • SHAMAL ABDULLAH AL-MUFFTI Dept. of Biology, College of Science, University of Duhok
Keywords: Toxoplasma gondii, Aborted women, ELISA, RT-PCR.


Background: Congenital toxoplasmosis is one of the most endemic parasitic infections that can lead to
clinical complications by transmission through mother to fetus transplacentally in aborted women.
Objectives: The aim of this study was to estimate the rate of toxoplasmosis among pregnant and aborted
women in Duhok/ Kurdistan Region-Iraq by using Enzyme-Linked ImmunoSorbent Assay(ELISA) and
detect the seropositive cases by Real-Time Polymerase Chain Reaction (RT-PCR) .
Materials and Methods: A total of 102 samples were collected from women admitted to obstetrics and
gynecology hospital in Duhok province. The data were collected through the period from January, 20th
2019 until December, 30th, 2019.Blood and placental samples from 60 aborted women with Bad Obstetric
History(BOH) and 42 placental samples from healthy normal pregnancies (as a control group). The
placental samples of normal pregnancies were tested by RT-PCR technique while aborted women’s blood
and placental samples were tested by both Enzyme-Linked ImmunoSorbent Assay(ELISA) and RT-PCR
Results: The results revealed a low rate of infection by congenital toxoplasmosis. Seropositivity to T.
gondii were 20/60 33.3% by ELISA, 25% IgM, 65% IgG and 10% both IgG and IgM whereas the retested
seropositive samples by RT-PCR only 5% showed positive results. The highest rate of infection was at 32-
38 age group, first trimester 85%, single abortion 35%, who inhibits rural area 80%,ingesting homemade
food 80%, and contact with animals(cats) 85%.
Conclusion: Real-time PCR-based technique is the most accurate and suitable for routine screening,
providing a rapid, sensitive, and quantitative way of detecting congenital toxoplasmosis in clinical
specimens, without any false-positive result that may help to a targeted treatment.


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Alaa M., Sukaina R., Neamah, Noor K. (2019).
Seroepidemiological study on Toxoplasma
gondii of aborted women at AL- Shatrah
hospital of AL- Shatrah district/ThiQar city,
Iraq. Journal of Kerbala University; 17(3):45-
Al-Hindi A., Lubbad A. (2009). Seroprevalence of
toxoplasmosis among Palestinian aborted
women in Gaza. Annals of Alquds medicine; 5:
Abdoli A., Dalimi A., Soltanghoraee H., Ghaffarifar
F. (2017). Molecular and genotypic
characterization of Toxoplasma gondii in
paraffin-embedded fetoplacental tissues of
women with recurrent spontaneous abortion.
International Journal of Fertility and Sterility;
Darweesh N., Hussein R., Salman S., Shaker M.
(2018). Immunological and Molecular study of
Toxoplasma gondii from aborted women in
Diyala, Iraq. Scientific Journal of Medical
Research; 2(6):75-82.
Mohammed L., Al-Janabi MS. (2019).
Seroprevalence of toxoplasmosis in aborted
women in Babylon province, Iraq. Medical
Journal of Babylon; 16:188-91.
Robert F., Dupretz P., Yvenou C., Quinio D., Poulain
P., Guiguen C., et al. (2010). Clinical
relevance of placenta examination for the
diagnosis of congenital toxoplasmosis.
Pediatric Infectious Disease Journal;
Hussein S., Elshemy A., Abdelmawgod M.,
Mohammed A. (2017). Seroprevalence of
Toxoplasma gondii among primigravida
women and their neonates in Sohag
governorate, Egypt. Journal of the Egyptian
Society of Parasitology; 47, 381-388. Pinon J., Dumon H., Chemla C., Franck J., Petersen
E., et al. (2001). Strategy for diagnosis of
congenital toxoplasmosis: evaluation of
methods comparing mothers and newborns
and standard methods for postnatal detection
of immunoglobulin G, M, and A antibody.
Journal of Clinical Microbiology; 39: 2267-
Rorman E., Zamir C., Rilkis I., Ben-David H. (2006).
Congenital toxoplasmosis prenatal aspects of
Toxoplasma gondii infection. Journal of
Reproductive Toxicology; 21: 458-472.
Mohammed T. (2011). Seroprevalence
of Toxoplasma gondii among pregnant women
in Baghdad city, Iraq. Iraqi Academic
Scientific Journal; 24:21–28.
Ajzenberg D., Yera H., Marty P., Paris L., Dalle F., et
al. (2015). Genotype of 88 Toxoplasma gondii
Isolates Associated with Toxoplasmosis in
Immunocompromised Patients and Correlation
with Clinical Findings. Journal of Infectious
Diseases; 199: 1155-1167.
Sugden K., Moffitt T., Pinto L., Poulton R., Williams
B., et al. (2016). Is Toxoplasma gondii
Infection Related to Brain and Behavior
Impairments in Humans? Evidence from a
Population-Representative Birth Cohort. PLoS
ONE 11(2); e0148435.
Aseel S., Sabeeha A., Abdul-Kareem A. (2019). PCR
detection of Toxoplasma gondii B1 gene in
women suffering from abortion. Journal of
Biotechnology Research Center; 13(1):12-15.
Wiengcharoen J., Chiabchalard R., Sukthana Y.
(2004). PCR technique for detecting
Toxoplasma gondii in animal amniotic fluid.
The Southeast Asian Journal of Tropical
Medicine and Public Health; 35(4):792-5.
Van de Ven E., Melchers W., Galama J., Camps W.,
Meuwissen J. (1991). Identification of
Toxoplasma gondii infections by BI gene
amplification. Journal of clinical
microbiology; 29(10):2120-2124.
Singh S. (2003). Mother to child transmission and
diagnosis of Toxoplasma gondii infection
during pregnancy. Indian journal of medical
microbiology; 21(2):69-76.
Fatin M., Mohammed A., Rafae Z., Mukhlif S.
(2018). Diagnostic study for Toxoplasmosis
by Serological test and PCR technique in
Kirkuk province, Iraq. Tikrit Journal of Pure
Science; 23(1): 19-25.
Badr M., Attia S., El-Sherbiny W., Abd-Allah
M., Hefny H., Salem A. (2016). Genetic
polymorphism of Toxoplasma gondii from
recently infected aborted Egyptian women.
Journal of the Egyptian Society of
Parasitology; 46(1):49-55.
Mousavi P., Mirhendi H., Mohebali M. (2018).
Detection of Toxoplasma gondii in acute and
chronic phases of infection in
immunocompromised patients and pregnant
women with real-time PCR assay using
TaqMan fluorescent probe. Iranian Journal of
Parasitology; 13(3):373-381.
Naqid I., Yousif S., Hussein N. (2019). Serological
study of IgG and IgM Antibodies to
Cytomegalovirus and Toxoplasma infections
in pregnant women in Zakho City, Kurdistan
Region, Iraq. Women's Health Bulletin; 6(4):8-
Manal A., SH Eassa, A.T.M. Al-saeed. (2016).
Detection of toxoplasmosis among women
with abortion using molecular and serological
tests in Duhok city, Iraq. Duhok Medical
Journal; 10(2):56-68.
Nash J., Chissel S., Jones J., Warburton F., Verlander
N. (2005). Risk factors for toxoplasmosis in
pregnant women in Kent, United
Kingdom. Epidemiology and Infection;
Sharifi K., Hosseini F., Tara F., Khaledi A., Shamsian
S. (2019). Diagnosis of Acute Toxoplasmosis
by IgG and IgM Antibodies and IgG Avidity
in Pregnant Women from Mashhad, Eastern
Iran. Iranian Journal of Parasitology;
Said B., Halsby K., O'connor C., Francis J., Hewitt
K., Verlander N., et al. (2017). Risk factors for
acute toxoplasmosis in England and
Wales. Epidemiology and Infection;
145(1):23-29. Scallan E., et al. (2015). An assessment of the human
health impact of seven leading foodborne
pathogens in the United States using
disability-adjusted life years. Epidemiology
and Infection; 143(13):2795-2804.
Mangen M., et al. (2015). Cost-of-illness and disease
burden of food-related pathogens in the
Netherlands, 2011. International Journal of
Food Microbiology; 196:84–93.
Fallah M., Rabiee S., Matini M., Taherkhani H.
(2008). Seroepidemiology of toxoplasmosis in
primigravida women in Hamadan, Islamic
Republic of Iran, 2004.The Eastern
Mediterranean Health Journal; 14(1):163-71.
Fenta D. (2019). Seroprevalence of Toxoplasma
gondii among pregnant women attending
antenatal clinics at Hawassa University
comprehensive specialized and Yirgalem
General Hospitals, in Southern Ethiopia. BMC
Infectious Diseases; 19:1056.
Mandour A., Mounib M., Eldeek H., Ahmad A.,
Abdel-Kader A. (2017). Prevalence of
congenital toxoplasmosis in pregnant women
with complicated pregnancy outcomes in
Assiut governorate, Egypt. Journal of
Advances in Parasitology; 4(1):1-8.
Abamecha F., Awel H. (2016). Seroprevalence and
risk factors of Toxoplasma gondii infection in
pregnant women following antenatal care at
Mizan Aman general hospital, bench Maji
zone (BMZ), Ethiopia. BMC Infectious
Diseases; 16(1):460.
Bamba S., Cisse M., Sangare I., Zida A., Ouattara S.,
Guiguemde R. (2017). Seroprevalence and
risk factors of Toxoplasma gondii infection in
pregnant women from Bobo Dioulasso,
Burkina Faso. BMC Infectious Diseases;
Tlamcan, Z., Lemkhenete Z., Lmimouni B. (2013).
Toxoplasmosis: The value of molecular
methods in diagnosis compared to
conventional methods. Journal of
Microbiology and Infectious Diseases;
Al-Dujaily K., Amir N. (2014). Combination of
ELISA and RT-PCR tests in the diagnosis of
toxoplasmic infection in aborted women and
congenitally infected infants. Journal of
Biotechnology Research Center; 8(3):44-47
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