DETECTION OF TOXOPLASMA GONDII AMONG WOMEN IN DUHOK CITY-KURDISTAN REGION OF IRAQ
Background: Congenital toxoplasmosis is one of the most endemic parasitic infections that can lead to
clinical complications by transmission through mother to fetus transplacentally in aborted women.
Objectives: The aim of this study was to estimate the rate of toxoplasmosis among pregnant and aborted
women in Duhok/ Kurdistan Region-Iraq by using Enzyme-Linked ImmunoSorbent Assay(ELISA) and
detect the seropositive cases by Real-Time Polymerase Chain Reaction (RT-PCR) .
Materials and Methods: A total of 102 samples were collected from women admitted to obstetrics and
gynecology hospital in Duhok province. The data were collected through the period from January, 20th
2019 until December, 30th, 2019.Blood and placental samples from 60 aborted women with Bad Obstetric
History(BOH) and 42 placental samples from healthy normal pregnancies (as a control group). The
placental samples of normal pregnancies were tested by RT-PCR technique while aborted women’s blood
and placental samples were tested by both Enzyme-Linked ImmunoSorbent Assay(ELISA) and RT-PCR
Results: The results revealed a low rate of infection by congenital toxoplasmosis. Seropositivity to T.
gondii were 20/60 33.3% by ELISA, 25% IgM, 65% IgG and 10% both IgG and IgM whereas the retested
seropositive samples by RT-PCR only 5% showed positive results. The highest rate of infection was at 32-
38 age group, first trimester 85%, single abortion 35%, who inhibits rural area 80%,ingesting homemade
food 80%, and contact with animals(cats) 85%.
Conclusion: Real-time PCR-based technique is the most accurate and suitable for routine screening,
providing a rapid, sensitive, and quantitative way of detecting congenital toxoplasmosis in clinical
specimens, without any false-positive result that may help to a targeted treatment.
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