MOLECULAR IDENTIFICATION AND INSECTICIDAL ACTIVITY OF Albifimbria verrucaria ISOLATED FROM CUCURBIT PLANTS AND SOIL IN IRAQ
Albifimbria (=Myrothecium) verrucaria (Alb. & Schwein.) L. Lombard & Crous. has been involved as
promising bio-pesticides for wide range of weeds and nematodes. Furthermore, has a promising
insecticidal activity because of its ability to produce many enzymes as lipase, protease and kinase that
have the ability to degrading the cuticles of insects. Seven isolates of A. verrucaria were isolated from
cucurbit plants and the soil. The identification was based on molecular (ITS-rDNA) and morphological
analyses. Laboratory bioassays to evaluate the insecticidal activity of A. verrucaria (cgh-38) against
different stages of squash beetle, Epilachna chrysomelina (F.) (Coleoptera: Coccinellidae) were conducted.
Three days post treatment, 87.5% mortality rate was obtained when the 1st instar larvae treated,
compared to 31.45 for 4th instar larvae. The highest mortality percent was 25 % for adults after 9 days of
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