MICROSCOPIC AND MOLECULAR IDENTIFICATION OF Anaplasma ovis IN SMALL RUMINANTS IN DUHOK PROVINCE IN KURDISTAN REGION OF IRAQ
In last ten years, there has been a developing enthusiasm for microscopic organisms from the genus
Anaplasma, particularly the species A. ovis. It is associated with the pathogenic action of these microscopic
organisms in livestock. Anaplasma ovis is a tick-borne obligate intracellular rickettsial bacterium that
causes anaplasmosis in domestic and wild small ruminants.
The samples of the present study were collected from small ruminants from inside seven distinct
regions (Akre, Simele, Zummar, Feshchapoor, Deraboon, Bajed Kandal,Karoda)of Duhok province, 389
(goats 75 and sheep 314) during the period of April and May 2018, blood sample were taken and thin
smear was formed, after Giemsa’s staining the slide is observed under microscope.
In this study used Giemsa stain for microscopic examination out of 389 animals 250 were found
positive for Anaplasma ovis infection with a prevalence rate of 64.26 % and 139 of them were negative
with a prevalence rate of 35.73 %.
According to the species of animals, the highest prevalence of A. ovis infection in animals by using
microscopic examination was 67.83 %, 213 positive sample from total 314 blood samples from sheep and
lowest prevalence was 49.33 %, 37 positive sample from total 75 blood samples from goats.
PCR analysis of 100 blood samples obtained from total 250 positive blood samples after DNA
extraction and measure of concentration and purity we used 2 primers that target major surface protein 4
(MSP4) in A. ovis genomic DNA.
The results of PCR test with major surface protein 4 primer was 83 samples positive from total 100
samples, According to the species of animals, the highest prevalence of A. ovis was 83.7 %, 72 positive
sample from total 86 blood samples from sheep and lowest prevalence was 78.5 %, 11 positive sample
from total 14 blood samples from goats.
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